1994-2019 All
Rights Reserved. Online Journal of Veterinary Research. You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright
treaties. Permission to use any of these pages in any
other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety
to OJVR publications. This article may be copied once but may not be,
reproduced or re-transmitted without the express permission of the editors.
OJVRTM
Online
Journal of Veterinary Research©
Volume
22 (5):417-424, 2018.
Prevalence
of Toxoplasma gondii
in abortive women in Iraq detected by ELISA and PCR.
Wafaa Ayad Al-Nuaimya
and Tareq Jafaar Al-Jandeelb.
aCollege of Science, bCollege of Veterinary Medicine, Al-Muthanna University, Al-Muthana, Iraq.
ABSTRACT
Al-Nuaimy WA, Al-Jandeel
TJ., Prevalence of Toxoplasma gondii in abortive
women in Iraq detected by ELISA and PC., Onl J Vet
Res., (5):417-424, 2018. Toxoplasmosis during
pregnancy is a significant risk factor for adverse pregnancy outcomes in
humans. One hundred twenty women from Al Muthanna,
Iraq who had had a single or repeat abortion were tested. ELISA produced sensitivity
of 75% and specificity 96.8% and RT-PCR produced similar results. By ELISA IgG incidence
was 17.5 % and by RT-PCR 20%. Most early miscarriages occurred during the 1st
trimester representing 50% of cases, 36.7% during the 2nd trimester and
13.3% during the 3rd trimester in stillbirth. We found 58(48.3%)
with single miscarriage, and 62(51.7%) with 2 or 3 or more recurrent
miscarriages. T. gondii
detected by qtPCR was associated (P < 0.02) with
miscarriages in the 1st trimester of pregnancy but by ELISA we found no significant differences
between trimesters. We find a high
prevalence for T. gondii in abortive women in
Iraq probably due to multiple transmission modes inducing early and recurrent
miscarriages.
Keywords
: T.gondii, early miscarriages, Late miscarriages,
Gestational age, qPCR.