©1996-2018. All Rights Reserved. Online
Journal of Veterinary Research . You may not store
these pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR. This article may be
copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJVRTM
Online
Journal of Veterinary Research©
Volume 18(6): 495-502,
2014. Redacted 2017.
Acute
phase responses in commercial broiler chickens experimentally infected with a
highly virulent Newcastle disease virus strain
Sobhan Firouzi1*, Hassan Nili1,
Keramat Asasi1, Saeed Nazifi1, Najmeh Mosleh1, Hassan Habibi2, Mitra Mohammadi1
1Avian Diseases Research Center,
Department of Clinical Studies, School of Veterinary Medicine, Shiraz
University, 2Department of Animal Science, College of Agriculture,
Persian Gulf University, Bushehr, Iran.
ABSTRACT
Firouzi S, Nili H, Asasi K, Nazifi S, Mosleh N, Habibi H, Mohammadi M., Acute phase responses in commercial broiler
chickens experimentally infected with a highly virulent Newcastle disease virus
strain, Onl J Vet Res., 18(6): 495-502, 2014. Acute phase responses (APRs) are plasma
proteins that increase or decrease in response to inflammation.
Inflammation-induced changes in groups of 15 four week old broilers vaccinated
twice with strain I-2 of NDV at days 28 and 35, and challenged intranasally with 0.05 ml allantoic
fluid containing 104.3 EID50/ml of a highly virulent NDV at day 42,
were monitored. Birds in other groups of 15 each were kept as positive and
negative controls. Sera samples were collected at days 47 and 52, and assayed
for inflammatory mediators (TNF-α and IFN-γ), acute phase proteins (haptoglobin (Hp) and serum
amyloid A (SAA)) and gangliosides (total sialic acid (TSA), lipid-bound sialic
acid (LBSA); and protein-bound sialic acid (PBSA)). Concentrations of HP, SAA, IFN-γ,
TNF-α, TSA, LBSA and PBSA in day 10 post challenge in vaccinated birds
were 1.12, 1.34, 1.33, 1.35, 2.07, 2.23 and 2.11 fold higher, respectively than
negative controls. Results suggested that APRs were useful
markers for prognosis and diagnosis of ND in poultry.
Key word: Acute phase protein, Broiler chicken,
Newcastle disease.