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OJVRTM

Online Journal of Veterinary Research©

 (Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

 Volume 25 (9):653-659, 2021.

Effect of silymarin supplement on ISA Brown layers feds aflatoxin contaminated feed

on egg quality and histopathology of liver, spleen and intestine.

 

Mushtaq Talib Abdulwahid1, Huda Hameed Alabbody2, Hussein Ali Rashid3

 

Department(s) 1Veterinary Public Health, College of Veterinary Medicine, University of Baghdad, 2Market Research Center and Consumer Protection, University of Baghdad, 3Ministry of Agriculture, Veterinary Directorate, Diyala, Iraq

 

ABSTRACT

 

Abdulwahid MT, Alabbody HH,  Rashid HA., Effect of silymarin supplement on ISA Brown layers feds aflatoxin contaminated feed on egg quality and histopathology of liver, spleen and intestine. Onl J Vet Res., 25 (9):653-659, 2021. Two groups of forty 217 day old 4-4.5kg ISA Brown layers were given feed with 14.6 ppb aflatoxin, with (1) or without (2) 0.5% silymarin/kg daily for 12 weeks. Forty controls were fed basal diet (3). At week 12 liver, intestine and spleen were excised for microscopy.  Compared with controls in birds given aflatoxin we found 66 to 93% reductions (P < 0.001) in egg weight and 30 to 70%  (P < 0.001 ) in shell quality. In layers supplemented silymarin we found no effect on egg quality. We observed liver congestion, cellular infiltrations, necrosis, apoptosis and hepatocyte degeneration, in spleen, coagulative necrosis, lymphopenia, hemorrhage and interstitial edema and in intestinal tissue severe mononuclear cellular infiltrations, apoptotosis, disrupted villi, atrophy and goblet cell hyperplasia. In those given silymarin we found no lesions except for mild mononuclear cellular infiltration and apoptosis in liver. Our results suggest that silymarin supplement may have reduced toxic effects of aflatoxin in layers

 

Keywords: Silymarin, aflatoxin, layers, histopathology.

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