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OJVRTM
Online Journal of Veterinary Research©
(Including
Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 30 (1): 32-41, 2026.
Serology
and lesions in broilers with avian H9N2 and bronchitis viruses.
Seifi S, Asasi
K, Mohammadi A, Shirzad MR,
Pourfallah M.
School (s) of Veterinary
Medicine, University of Mazandaran, Shiraz University, Shiraz, Iran.
ABSTRACT
Seifi S, Asasi K, Mohammadi A, Shirzad MR and Pourfallah M. Serology
and lesions in broilers with avian H9N2 and bronchitis viruses., Onl J Vet Res, 30 (1): 42-49, 2026. Avian H9N2 influenza virus is mostly slightly
pathogenic but cases with high mortality have been observed. We excised
tracheal samples from broilers with respiratory disease and high mortality for viruses
by RT – PCR. For serology, inoculums from trachea from broilers with H9N2
AIV, bronchitis or both viruses, we inoculated
0.2 ml intranasally groups of twenty 21 day old birds
and monitored clinical signs, feed intake, mortality and gross lesions. Birds
were bled on 0, 10 and 20 days after inoculation for sera antibodies to H9 by hemaglutinin and bronchitis virus by ELISA. By AI Hematoglutinin tests. compared with controls, we found that chicks infected with AIV
and bronchitis virus, antibody increased (P < 0.05) 43% by day 10 and 73% by
day 20, with AIV 24 and 67% whereas with Bronchitis virus declined 24 and 100%.
By ELISA same results were AIV + IBV 63 and 75%, AIV -12 and -55%, IBV 71 and 78%, respectively.
Key words:
Avian Influenza Virus, Infectious Bronchitis Virus, Co- infection
.
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