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OJVRTM
Online Journal of Veterinary Research©
Volume 23 (12):1184-1187, 2019.
Colony-PCR for direct extraction of DNA from Corynebacterium pseudotuberculosis.
Taha Yassin, Ghani Al-Badrawi
College of
Veterinary Medicine, University of Baghdad, Iraq
ABSTRACT
Taha Yassin, Ghani Al-Badrawi,
Colony PCR for direct extraction of DNA from Corynebacterium pseudotuberculosis, Onl J Vet Res., 23 (12):1184-1187,
2019. Authors describe a
method to extract DNA directly from Corynebacterium
pseudotuberculosis for PCR. C. pseudotuberculosis was cultured anaerobically on solid
media incubated at 37C for 48-72 hours. C. pseudotuberculosis colony was
added to PCR master mix mixture instead of DNA template extract. We then amplified
the PCR product on agarose gel along with a DNA marker producing clear wide compact
electrophoretic DNA bands. We find this PCR assay could identify Corynebacterium pseudotuberculosis
directly without the need to extract DNA from bacterial isolates.
KEY-WORDS: PCR,
C. pseudotuberculosis