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OJVRTM
Online Journal of Veterinary Research©
Volume 16 (4): 284-289, 2012. Redacted 2017.

Anthrax vaccine 34F2 Sterne strain growth in nutrient agar and soyabean casein digest agar

 

S.Parthiban1*, Lancy Mammen2 S.R.Kalaivani3 V.Sundrapandian4 and A.Habibur Rahman5

 

Institute of Veterinary Preventive Medicine, Ranipet. 

 

ABSTRACT

 

Parthiban S, Mammen L, Kalaivani SR, Sundrapandian V, Rahman AH., Anthrax vaccine 34F2 Sterne strain growth in nutrient agar and soyabean casein digest agar, Onl J Vet Res., 16 (4): 284-289, 2012. The most widely used strain for anthrax vaccine production is 34F2 Sterne strain. We describe a suitable solid media for the cultivation of 34F2 Sterne strain for large scale vaccine production. Seed material (34F2 Sterne strain) was cultured in 70 Roux flasks each containing Nutrient agar and Soyabean casein digest agar separately and allowed for sporulation. The spores were washed with sterile saline and the pure spore suspensions were pooled and weighed separately. The weight of the spore suspension was 1.801 kg and 1.830 kg for Nutrient agar and Soyabean casein digest agar respectively. Twice the weight of the spore suspension, sterile glycerol was added and kept for stabilization at 20°C for 21 days to destroy vegetative bacteria and weak spores. The number of live spores was 1:250 in 1 ml of 10-7 dilution for both Nutrient agar and Soyabean casein digest agar grown spore suspensions.

 

Key words: Anthrax, 34F2 Sterne strain, Growth, Nutrient agar, Soyabean casein digest agar.

 

 

 

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