MAIN


©1996-2016.  All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the  before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJBTM

Online Journal of Bioinformatics ©

Volume 16(3): 303-317, 2015.


In silico analysis of arsenate reductase gene in biological systems

Sarita Tiwari1, Bijaya Ketan Sarangi1 Jeya Nasim2, Dinesh Yadav2

 

1National Environmental Engineering Research Institute, Council of Scientific and Industrial Research, Nehru Marg, Nagpur 2Department of Biotechnology, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh, India.

 

ABSTRACT

 

Tiwari S, Sarangi BK, Nasim J, Yadav D., In silico analysis of arsenate reductase gene in biological systems, Onl J Bioinform., 16(3): 303-317, 2015. ArsC cistron operon reduces arsenate As(V) to arsenite As(III), a primary step in arsenic (As) detoxification. It is presumed that this mechanism has evolved among prokaryotic bacteria and eukaryotic yeast and plants. 174 arsenate reductase (AR) protein sequences from 5 different species retrieved from NCBI were analyzed for similarity, evolution pathway, homology search, multiple sequence alignment, phylogenetic tree construction, motif and functional divergence. Conserved regions at different stretches of the sequences revealed the identity of AR and the phylogenetic tree showed 5 major cluster source organisms Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae, Arabidopsis thaliana and Pteris vittata. Variability in the distribution of the six motifs was observed along with unique motif 4 and motif 6 exclusively for S. aureus and P. vittata respectively. The  coefficient of functional divergence was found to be <1, indicating prevalence of site-specific selective constraints leading to functional evolution after diversification.

 

Keywords: Arsenic, arsenate reductase, evolution, phylogenetic relation


MAIN

 

FULL-TEXT(SUBSCRIPTION)