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OJVRTM
Online
Journal of Veterinary Research©
Volume 18(12): 914-922,
2014. Redacted
2018.
Experimental focal cerebral ischemia and reperfusion
by temporary occlusion
of the middle cerebral artery in female Wistar rats
Danilo Ferreira Rodrigues1*, Fernanda
Figueiredo Mendes1,Taís Andrade Dias1, Ashbel Schneider
da Silva2, Tales Dias do Prado3,
Luiz Antônio Franco da Silva1, Eugênio
Gonçalves de Araújo1
1School
of Veterinary and Animal Science, Federal University of Goiás,
Goiânia, Goiás, Brazil, 2Veterinarian, 3Rio Verde University, Rio
Verde, Goiás, Brazil.
ABSTRACT
Rodrigues DF, Mendes FF, Dias TA, Silva AS, Prado
TD, Silva LAF, Araújo EG., Experimental focal
cerebral ischemia and reperfusion model by temporary occlusion of the middle
cerebral artery in female Wistar rats, Onl J Vet Res., 18(12): 914-922, 2014. Ischemic cerebrovascular
diseases are major causes of disability and death worldwide. A simple but novel
surgical technique to induce focal cerebral ischemia and reperfusion in 12
female Wistar rats is described. Endovascular
surgical 0 polypropylene was catheterized through the main branch of the
left carotid artery. After penetrating 1cm and occluding the common
carotid artery, the catheter was removed and the polypropylene was guided
laterally toward the internal branch of the common carotid artery 1.8 cm from
the point of bifurcation of the common carotid and middle cerebral artery. The
cerebral artery was occluded for 30 minutes to maintain ischemia. The polypropylene was then removed to allow
reperfusion and clinical assessment for 24 hours. Brain sections were assessed
microscopically for focal cerebral ischemia followed by reperfusion. Results
showed that ischemia and reperfusion occurred in all rats. The technique was
performed easily and reproducible when compared with current similar methods
but requires prior training in microsurgery, and histo-chemical
and imaging evaluations. A video and Illustrations of the technique are shown.
Key
words: carotid artery,
cerebrovascular accident, endovascular yarn, focal cerebral ischemia and
reperfusion.
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