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OJVRTM

 Online Journal of Veterinary Research©

 

Volume 18(5): 391-397, 2014. Redacted 2017.


Purification of ISS protein from avian pathogenic Escherichia coli strain χ1378 by affinity chromatography

 

B. Nayeri1, A. Derakhshandeh2, T. Zahraei Salehi1, S. Gholami2, E. Ebrahimzade1, Z. Naziri 2

 

1Department(s) of Microbiology, Faculty of Veterinary Medicine, University of Tehran and 2Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

 

ABSTRACT

 

Nayeri B, Derakhshandeh A, Zahraei Salehi T, Gholami S, Ebrahimzade E, Naziri Z., Purification of ISS protein from avian pathogenic Escherichia coli strain χ1378 by affinity chromatography, Onl J Vet Res., 18(5): 391-397, 2014. Colibacillosis due to avian pathogenic Escherichia coli affects poultry worlwide. A major virulence factor is increased serum survival (ISS) gene resistant to the complement system. ISS protein of virulent E coli from avian colibacillosis was expressed and purified using gene specific primers to generate a 359 bp product. ISS protein was analyzed by SDS-PAGE, affinity chromatography and Xa factor for purification and separation of the recombinant protein. SDS-PAGE and western blot analyzes confirmed purity and antigenicity of the protein showing a 8 kDa band protein.

 

KEY-WORDS: ISS protein, E Coli, Chromatography


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