MAIN


©1996-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


 

OJVRTM

Online Journal of Veterinary Research©

Volume 20(8):507-513, 2016.


Susceptibility of avian Trichomonas strains to metronidazole

 

Mohammad Rajabloo1, Seyed Mostafa Razavi1, Gholamreza Hatam2, Fatemeh Nejati1

 

1Department of Pathobiology, School of Veterinary Medicine, Shiraz University, 2Basic Sciences and Infectious Diseases Research Center, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran

 

ABSTRACT

 

Rajabloo M, Razavi SM, Hatam G, Nejati F., Susceptibility of avian Trichomonas strains to metronidazole, Onl J Vet Res., 20(8):507-513, 2016. Genetic variations are possible causes for different sensitivity patterns of protozoa against antiprotozoal compounds. Eleven isolates of T. gallinae identified based on ITS1/5.8S/ITS2 region and 18S rRNA gene including T. gallinae, T. tenax and T. vaginalis strains were subjected to In vitro inhibition by metronidazole after 24h incubation under aerobic and anaerobic conditions. Minimum lethal concentration (MLC) was set at ≥50 µg/ml under aerobic and ˃12.5 µg/ml under anaerobic condition. MLCs of 8 T. tenax-strains (Type II of ITS1/5.8S/ITS2 region and type I of 18S rRNA gene) varied 1.56-6.25 µg/ml  and those of other T. tenax (Type III of ITS1/5.8S/ITS2 region and type X of 18S rRNA gene) was 1.56 µg/ml under aerobic condition. The MLCs of T. tenax-like strains were less than those of T.gallinae-like strains in both studies. Under aerobic conditions MLC’s for T. gallinae-like isolates MR28 and MR30 were 12.5ug/ml, and higher compared with other T. tenax isolates (0.8 to 6.25 µg/ml). Similar results were achieved under anaerobic condition. Results suggest that T. gallinae-like strains were more resistant to metronidazole than T.tenax-like strains.

 

Keywords: Drug susceptibility, Metronidazole, T.gallinae-like strain, T. tenax-like strain.


 

MAIN

 

FULL-TEXT(SUBSCRIPTION OR PURCHASE TITLE $25USD)