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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 30 (9): 520-530, 2026.

Pasteurella multocida biofilm grown on soya broth and bentonite

 

Rajagopal R, Krishnan Nair G, Mini M, Leo Joseph.

 

 

College of Veterinary and Animal Sciences, Kerala, India.

 

ABSTRACT

 

Rajagopal R, Krishnan Nair G, Mini M, Leo Joseph., Pasteurella multocida biofilm grown on soya broth and bentonite. Onl J Vet Res., 30 (9): 520-530, 2026. The organism adhered to bentonite with an average peak count of 1.54 × 106 CFU g-1 of BC, 3 days after inoculation, whilst planktonic cells reached a peak count of 8.10 × 108 CFU mL-1, 1 day post inoculation. The biofilm colony on agar medium appeared discernably different from planktonic colonies, though it reversed on subsequent subcultures. Biofilm cells appeared extremely capsulated.

 

KEY WORDS : Biofilm,  Pasteurella multocida, bentonite clay, capsular polysaccharide, Maneval staining, phenol sulphuric acid method.

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