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OJVRTM
Online Journal of Veterinary
Research©
Volume 21(8):440-452, 2017.
In Vivo 3-D Bioceramic scaffold for bone repair in
male rabbits.
Bahaa Fakhri Hussein
Department of Anatomy and Histology, College of Veterinary Medicine,
University of Baghdad, Baghdad, Iraq
ABSTRACT
Hussein BF., In Vivo 3-D Bioceramic scaffold for bone repair in male rabbits, Onl J Vet Res., 21(8):440-452, 2017. A scaffold of gelatine, hydroxyapatite (Ca10(PO4)6(OH)2)
nano-particle powder and dextrin seeded by stem cells
for radial bone repair is described. The scaffold was cross-linked and
sterilized by exposure to 25Kgy gamma radiation. Under
anesthesia, a 2cm critical size defect was created on
radial bone of five 1 to 1.5y old male rabbits and replaced with the scaffold,
but without seeding of osteoblast. Controls were subjected to same procedure but
not implanted. Tramadol hydrochloride and streptopenicillin
were given 3-5d after implant. Radiographs were taken
1d, 1 and 3mth after implant to monitor formation of new bone. Blood was taken
for calcium and alkaline phosphatase (ALP), before
and 7, 14, 21, 28, 35, 60 and 90d after implant. One rabbit with implant was
euthanized at 90d for microscopy. Scaffolds were evaluated by X-ray
diffraction, Fourier-Transform infrared spectroscopy, scanning electron
microscopy and compression mechanical tests. After 90d, implants revealed bone
formation with the scaffold completely dissolved or absorbed. Bone appeared
centripetal with a network of interconnected pores. Blood ALP and calcium
appeared higher (P<0.05) in implanted rabbits. There were no signs of
inflammation, pain or adverse tissue reaction.
Key word: Tissue Engineering, Bioceramic Scaffold, Rabbits.
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