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OJVRTM
Online
Journal of Veterinary Research©
Volume 23 (10):989-998, 2019.
Formation of
Pasteurella multocida
biofilm on bentonite.
Rajagopal R1*,
Krishnan Nair G1 , Mini M2, Leo
Joseph3
1,2Department of Veterinary Microbiology, College of
Veterinary and Animal Sciences and 3Kerala Agricultural University
Poultry Farm, College of Veterinary and Animal Sciences, Kerala, India.
ABSTRACT
Rajagopal R, Krishnan NG, Mini M,
Joseph L., Formation of Pasteurella multocida biofilm on bentonite, Onl
J Vet Res., 23 (10):989-999, 2019. Formation of
Pasteurella multocida
biofilm was assessed in tryptone soya broth
supplemented with bentonite. The organism adhered to bentonite with an average
peak count of 1.54 × 106 CFU g-1 of BC, 3 days after
inoculation, whilst planktonic cells reached a peak count of 8.10 × 108
CFU mL-1, 1 day post inoculation. The biofilm colony on agar medium
appeared discernably different from planktonic colonies, though it reversed on
subsequent subcultures. Biofilm cells appeared extremely capsulated.
KEY WORDS : Biofilm, Pasteurella multocida, bentonite clay, capsular polysaccharide, Maneval staining, phenol sulphuric
acid method.
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