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OJVRTM
Online Journal of Veterinary
Research©
Volume 24 (6):312-327, 2020.
Effect of selenium loaded Ribes nigrum nanoparticles on genetic markers in male rats with
D-galactose induced toxicity.
Masar Jabbar Jary Al-Kurdy1
and Khalisa Khadim Khudair2
1Nursing,
Technical Institute in Al-Diwaniyah, AL-Furat AL-Awsat Technical University, 2Department of
Physiology, biochemistry and Pharmacology, College of Veterinary Medicine,
University of Baghdad, Iraq.
ABSTRACT
Jary Al-Kurdy MJ, Khudair KK., Effect of selenium loaded Ribes
nigrum nanoparticles on genetic markers of male rats
with D- galactose induced toxicity, Onl J Vet Res., 24 (6):312-327, 2020. Authors report effect of selenium nanoparticles loaded with black
currant (Ribes nigrum) extract
on serum caspase3, chromosomes, mitotic index, liver tunel
cells and apoptosis in Wistar male rats given
150mg/kg D-galactose IP daily for 56 days. We generated 18-50nm spherical
crystallite nanoparticles identified by UV spectroscopy, electron microscopy and
X–ray diffraction. Groups of 8 rats each were then injected 150mg/kg D-galactose IP dissolved in
0.9% saline daily for 8 weeks (Group 1), gavaged
1mg/kg nanoparticles (2) or given both (3). Controls were given saline IP and orally (4). At days 14 and 56, cardiac blood was taken for caspase-3
and femur bone
excised for chromosomal
aberration and mitotic indices, and livers for DNA fragmentation and apoptosis. Compared with controls, we found significant (P < 0.05)
declines in serum caspase (~-10 to -17%), and chromosomal acentric aberrations (~-4
fold) in rats given nanoparticles with or without galactose. In rats given only
galactose we found large increases in serum caspase-A (~18%) chromosomal abberations (~12 fold) and mitotic indices (~34%). We found
no tunnel liver cells in rats given selenium loaded Ribes nigrum
nanoparticles with or without D galactose but present throughtout
in those given only D-galactose. Results suggest that selenium loaded Ribes nigrum nanoparticles nanoparticles may reduce D- galactose genotoxicity in rats
and exert anti-oxidants effects.
Key Words: Selenium,
nanoparticles, black current, apoptosis, liver.