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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 29 (5): 306-313, 2025.


Tyrosine hydroxylase immunoreactivity in bladder of cats with cystitis.

 

Reche A, Buffington TCA,  Hagiwara MK, Daniel AGT.  

 

 aDepartment of Medical Clinics Faculdade de Medicina Veterinaria, Universidade de Sao Paulo, Brazil b. Departmet of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus, Ohio-USA.

 

ABSTRACT

 

Reche A, Buffington TCA, Hagiwara MK, Daniel AGT., Tyrosine hydroxylase immunoreactivity in bladder of cats with cystitis., Onl J Vet Res., 29 (5): 306-313, 2025. We report identification and quantity sympathetic nervous fibers in urinary bladder of 6 healthy cats and 6 with interstitial cystitis without clinical signs for 6 months before study. All cats were euthanized and immediately perfused transcardially with Krebs-Ringer and 4% paraformaldehyde solutions. Urinary bladders were collected and frozen at -70C. Tissue slides were processed for immunohistochemistry by avidin-biotin to localize neuronal tyrosine hydroxylase. Compared with controls, in cats with cystitis we observed increased sympathetic nervous fibers in lamina propria and muscular layer of urinary bladder which may be induced by neurogenic inflammation.

 

KEY WORDS: interstitial cystitis, cats, tyrosine hydroxylase, urinary bladder.


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