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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
29
(6): 369-375, 2025.
Effect of cryo-preservation
on Boer goat spermatozoa.
Madurantakam SN, Masilamani
JE
Semen Bank, Department of Animal
Genetics & Breeding, Madras Veterinary College, Tamilnadu
Veterinary and Animal Sciences University Chennai – 600 007.
Madurantakam SN, Masilamani
JE., Effect of cryopreservation on Boer goat spermatozoa, Onl
J Vet Res., 29 (6): 369-375, 2025. We report effect of cryo-preservation
on Boar goat spermatozoa. Twenty seven semen samples were collected from 3
adult Boer grade bucks with 2 samples collected twice weekly evaluated for
volume, color, consistency, mass activity, sperm concentration, motility,
mortality and abnormalities. Sperm was cryopreserved in tris-egg
yolk based dilutor containing 7 percent glycerol. After 4 hours of
equilibration at 5˚C, the samples were packaged in 0.25 ml French mini
straws and frozen by exposure to liquid nitrogen vapour
horizontally. The frozen straws were plunged in liquid nitrogen for final
freezing and storage at - 196˚C.
Hamilton-Thorne integrated visual optical system was used to analyze
samples. Cryo-preservation reduced (P < 0.01)
motility ~38%, progressive motility ~39%, path velocity 16%, progressive
velocity ~15% and track speed ~16%. beat cross
frequency, straightness and linearity were not affected. However, variations
found between bucks in post-thaw semen for beat cross frequency and linearity
were highly significant (P ≤ 0.01).
KEY WORDS: Goat, Spermatozoa, Kinematics,
Cryopreservation, CASA.