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OJVRTM
Online Journal of Veterinary Research©
24(7): 438-448, 2020.
Bone marrow stem
cell implant for articular cartilage defects in Guinea pigs.
Davood Mehrabani1,2,3,4,
Mohammad Reza Sadraei5*, Nader Tanideh1, Mehrzad Lotfi6, Shahrokh
Zare1, Mina Rabiee1
1Stem
Cell and Transgenic Technology Research Center, 2Burn and Wound
Healing Research Center, 3Comparative and Experimental Medicine
Center, 6Imaging Research Center, Department of Radiology, Shiraz
University of Medical Sciences, Shiraz, Iran. 4Li Ka Shing Center for Health
Research and Innovation, University of Alberta, Edmonton, AB, Canada.
5Department of Biology, Science and Research Branch, Shiraz Islamic Azad University,
Fars, Iran
*Correspondence:: mreza_sadraie@yahoo.com
ABSTRACT
Mehrabani D, Sadrae MR, Tanideh N, Lotfi M, Zare S, Rabiee M., Bone marrow
stem cell implant for articular cartilage defects in Guinea pigs, Onl J Vet Res., 24(7): 438-448, 2020. Mesenchymal stem cells can differentiate into
cartilage tissue. We describe effect of bone marrow-derived stem cell implant
on full-thickness femoral articular cartilage defect in Guinea pigs. Under
anesthesia, groups of 7 animals each were induced an articular defect and then
implanted with stem cells. For sham guinea pigs, the anterior
cruciate ligament was cut to induce osteoarthritis for 12 weeks but without
cell implant. Controls were normal. Before and after cell therapy, femur-tibia
joints were evaluated histologically and by radiography. Spindle shape stem
cells exhibited plastic adherence, osteogenic and adipogenic
differentiation with doubling time of 65.6h. RT-PCR revealed stem cell
expression of CD44 and CD90 but absence of CD34 suggesting mesenchymal
properties. Radiography at 12 weeks in shams revealed joint deformation,
osteophytes, loss of joint space and knee osteoarthritis whereas in those
implanted stem cells joint were healed. Similarly microscopy of joint tissue
showed bone growth. Findings suggest that bone marrow stem cell implants may
enhance knee cartilage repair in guinea pigs.
Key words: Bone marrow, Mesenchymal stem cells, Cartilage defect,
Osteoarthritis, Healing, Guinea pig.
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