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OJVRTM
Online Journal of Veterinary
Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29
(10): 612-620, 2025.
Plasmid vector for recombinant bovine herpesvirus
De Souza VP, Dellagostin OA, Roehe PM.
Centre of Veterinary Research Desidério Finamor, Eldorado
do Sul, RS, Centre of Biotechnology,
Federal University of Pelotas, Department of Microbiology, Virology
Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Souza
VF, Dellagostin OA, Roehe PM. Plasmid
vector for recombinant bovine herpesvirus, Onl J Vet Res., 29 (10): 612-620,
2025. We report PCR-based plasmid vector for recombinant Bovine
Herpesvirus type 5 (BHV-5) by amplify the flanking sequences (5’ and 3’
regions) of the glycoprotein E (gE) gene of BHV-5 resulting in a gE’ vector
after sequencial subcloning steps, and also amplified the enhanced green
flourescent protein (EGFP) reporter gene, subcloned between 5’ and 3’ gE DNA
fragments resulting in a gE-EGFP vector. This vector can be used for
the production of recombinant BHV-5 to improve the study about deleted viral
samples. Moreover, this protocol can also be adopted for construction of
plasmids for production of several recombinant DNA viruses.
KEYWORDS:
PCR, Cloning, Plasmid, EGFP, BHV-5, Bovine herpesvirus.