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OJVRTM
Online Journal of
Veterinary Research©
12 (2) :38-46,
2008. Editors note: Extensively redacted 2017.
PCR for detection of Bifidobacterium spp in broiler intestine.
Seidavi AR1*, Mirhosseini SZ2,3, Shivazad M4,5,
Chamani M5, Sadeghi
AA5, Pourseify
R3
1Animal Science Department(s) of Islamic Azad, 2Guilan, 4Tehrana
and 5Islamic Azad Universities, and 3Agriculture Biotechnology Research Institute, Iran
ABSTRACT
Seidavi AR, Mirhosseini
SZ, Shivazad M, Chamani M, Sadeghi AA, Pourseify R., PCR for detection of Bifidobacterium spp in broiler
intestine, Onl J Vet Res., 12 (2) :38-46,
2008. A PCR was developed for rapid detection of bifidobacterium
spp. in broiler intestine samples using two 16S rRNA
gene Bifidobacterium genus-specific primers, Bif164-f and Bif662-r. No
differences were found between the duodenum, jejunum, ileum and cecum of
broilers at 4, 14 and 30 days of age. Bifidobacterium spp. was
detected in 75% of birds. 72, 78 and 75.0% samples were positive for naturally
occurring bifidobacterium spp
at 4, 14 and 30 days, respectively. This PCR allowed a rapid, highly specific
and reproducible detection of Bifidobacterium Spp. genus in poultry gut
samples.
Key words: broiler chicken, bifidobacterium,
gut, PCR, age, detection
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