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OJVRTM
Online Journal of Veterinary
Research©
Volume 17 (4): 159-166, 2013. Redacted 2017.
Detection of avian Metapneumovirus
infection in broilers by nested RT-PCR
Hesami G1, Seyfi Abad Shapouri MR, DVM PhD,2 Mayahi
M. DVM PhD3
2Departments of Pathobiology and Clinical
Sciences, 1School of
Veterinary Medicine, Shahid Chamran
University, Ahwaz, Iran.
ABSTRACT
Hesami G, Seyfi Abad Shapouri MR, Mayahi M., Detection of avian Metapneumovirus
infection in broilers by nested RT-PCR, Onl J Vet
Res., 17 (4): 159-166, 2013. Avian Metapneumovirus
(aMPV) is the casual agent
of Turkey Rhinotracheitis (TRT) which also causes a
respiratory infection in chickens, resulting in Swollen Head Syndrome. aMPV infection in chicken flocks
gives rise to considerable economic losses. aMPV infections were detected by RT-PCR in broiler
flocks in Ahwaz, Iran. Fifty broiler flocks were sampled at slaughter via
tracheal swabbing. RNA was extracted and RT-PCR based on viral N gene sequence
specific primers (Nd and Nx)
was performed. Positive results were confirmed by a semi-nested PCR. aMPV
was detected in 28% of samples. We report prevalence of aMPV
infections and describe a novel semi nested PCR to confirm Nd/Nx in broilers.
Keywords: Avian Metapneumovirus,
N gene, RT-PCR, Semi-nested PCR, Broiler flocks, Ahwaz