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OJVRTM
Online Journal of Veterinary Research©
Volume 17(9): 479-489, 2013. Extensively redacted 2017.
Molecular
cloning and expression of S1 glycoprotein gene of infectious bronchitis virus
(IBV) isolate IRFIBV32 in Lactococcus lactis
Hosseinian
SA1, Asasi K1, Hosseini A2,
Eskandari MH3, Karimi
Madab M1.
1Poultry Diseases Research Center, 2Immunology
Division, Department of Pathobiology, School of Veterinary Medicine, and 3Department
of Food Science and Technology, College of Agriculture Shiraz University,
Shiraz, Iran
ABSTRACT
Hosseinian SA, Asasi
K, Hosseini A, Eskandari MH, Karimi
Madab M., Molecular cloning and expression of S1
glycoprotein gene of infectious bronchitis virus (IBV) isolate IRFIBV32 in Lactococcus lactis, Onl J Vet Res., 17(9): 479-489, 2013. Avian infectious bronchitis virus (IBV) is a highly infectious pathogen
in chickens causing acute, highly contagious respiratory disease. S1
glycoprotein protein in IBV is pathogenic. Lactococcus lactis bacteria can be used to create mucosal recombinant vaccines. In this study, S1 glycoprotein gene of infectious bronchitis virus
isolate IRFIBV32 was cloned into L. lactis NZ9000.
pNZ8110-S1/S and pNZ8148-S1/C plasmids were used for
cytoplasmic and secretory protein production in E. coli MC1061. The plasmids were electrotransformed
to L. lactis NZ9000. Genes sequence was verified by PCR, digestion
and sequencing and intra and extracellular expression of S1 protein was
confirmed by SDS-PAGE.
Keywords: Molecular cloning,
Expression, S1 protein, IRFIBV32, L. lactis
NZ9000.