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OJVRTM
Online Journal of Veterinary
Research©
Volume 24 (3):191-197, 2020.
Effect of high fat diet on
intake, energy, insulin resistance, glucose tolerance and brown adipose tissue microscopy in
C57BL/6 mice.
Paulo
Henrique Sampaio da Silva¹, Cecília Ferreira Amaral
Silva¹, Márcio Botelho de
Castro²,
Eduardo
Maurício Mendes de Lima¹*, André Rodrigues da Cunha Barreto-Vianna³
¹Department(s) of Veterinary Anatomy, ²Veterinary Pathology, Faculty
of Agronomy and Veterinary Medicine, University of
Brasília and ³Department of
Veterinary Anatomy, Federal University of Paraná-Palotina Sector, Brazil. *Corresponding Author:limaemm@hotmail.com
ABSTRACT
Sampaio da Silva
PH, Amaral Silva CF, Castro MB , Mendes de Lima EM, Barreto Vianna AR, Effect of high fat diet on intake, energy, insulin
resistance, glucose tolerance and brown adipose tissue microscopy in C57BL/6
mice, Onl J Vet Res., Volume 24 (3):191-197, 2020. Brown
Adipose Tissue (BAT) generates heat, being rich in mitochondria and vessels, but normally with low fat levels. However, in
obesity, fat accumulates in BAT cells which
can then induce fibrosis. We report effect of high fat (HF) diet on intake, energy, insulin resistance,
glucose tolerance and BAT histology in C57BL/6 mice. Groups
of 10 mice each were fed HF diet
of 21kJ/g energy and control
standard diet with 15kJ/g for 14 days. In those given HF we found increased mean body mass
(~22%), feed intake (28%), plasma
glucose (30%),
intraperitoneal (42%) and oral insulin
resistance (~38%) but a
decline in insulin sensitivity (~-44%). BAT volume was
~54% in mice given HF diet and ~81%
in controls whereas white fat content was much higher
in those given HF (33%)
compared with controls (5.7%). Vascularization in mice
given HF was ~4.5% compared with controls (7.4%).
We found increased
fibroblast (8.4%) response
in mice given high fat diet compared with controls (6.1%) suggesting inflammation. Significance for mean differences ranged P
< 0.005-0.0001. The findings suggest that high fat diets may induce inflammation and whitening in brown adipose tissue, with insulin resistance and glucose
intolerance, but without fibrosis in mice.
Keywords: Brown adipose tissue,
inflammation, mice, fibrosis.
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