©1996-2018. All Rights Reserved. Online
Journal of Veterinary Research . You may not store
these pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR. This article may be
copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJVRTM
Online Journal of Veterinary Research©
Volume
16(1):16-25, 2012. Extensively redacted 2017.
Humoral and cellular immune responses in goats
exposed to recombinant cells expressing Omp 34kDa Brucella melitensis
gene.
Y. Puspitasari,
M. Zamri-Saad, M.Y. Sabri,
A.B. Zuki
Department
of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang,
Malaysia
ABSTRACT
Puspitasari Y, Zamri-Saad M, Sabri
MY, Zuki AB., Humoral and cellular immune responses
in goats exposed to recombinant cells expressing the Omp
34kDa Brucella melitensis gene, Onl
J Vet Res, 16(1): 16-25, 2012 The ability of
recombinant cell expression of 34kDa Brucella melitensis
gene to stimulate humoral and cell-mediated immune responses in goats is described. Three
groups of 5 healthy 1 yr old goats each, without B. melitensis
vaccinations, were injected IM 2ml 109
cfu/ml inactivated recombinant cells, 2ml 109
cfu/ml killed B. melitensis
and 2ml sterile PBS (controls),
respectively. Booster doses were given 2 weeks after the first injection. Serum
and blood antibody levels were determined with ELISA and cell-mediated immune
responses of CD4+ and CD8+ T-cells by immunofluorescence.
Goats exposed to recombinant or whole-cell antigen showed higher IgG levels
than unvaccinated goats throughout the entire 8-week study period. Higher
antibody levels occurred within 1 week after vaccination and titers increased
after booster injections. In contrast, the cellular
immune response, particularly the percentage of CD4+ and CD8+
T-cells did not increase after the first and second exposures to the
recombinant cells but
did at the 4 and 5th week. Results demonstrate
that despite the high antibody levels of IgG,
exposure to recombinant cells expressing the Omp 34 kDa gene of B. melitensis strain
293, delayed systemic CD4+ and CD8+
T-cells responses for a short period.
Keywords: immune responses, humoral, cellular,
recombinant cells, Brucella melitensis
FULL-TEXT (SUBSCRIPTION OR
PURCHASE ARTICLE)