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OJVRTM
Online
Journal of Veterinary Research©
Volume 24(8): 475-482,
2020.
Survey of Brucella
canis in dogs in a small animal hospital setting.
Sina
sakhaii Far, Seyed Javid Aldavood, Fatemeh Gheitanchi, Sana Razavi Fard
Department
of Small Animal Internal Medicine, Faculty of Veterinary Medicine, University
of Tehran
ABSTRACT
Far
SS, Aldavood SJ, Gheitanchi
F, Fard SR., Survey
of Brucella canis in dogs in a small animal hospital
setting, Onl J Vet Res., 24(8): 475-482,
2020. Brucella canis causes
brucellosis and reproductive failure in dogs but can also infect humans causing fever, chills, malaise, peripheral lymphadenomegaly, and splenomegaly. However, B. canis infection in
dogs is underrecognized by serologic data,
transmission patterns, and regulations in context of a zoonosis. Therefore development of appropriate diagnostic
methods in dogs is necessary. Various methods have been developed to diagnose B.canis including serology and polymerase chain reaction (PCR). We
tested 47 female and 73 male dog blood samples referred to the Small Animal Hospital of the Faculty
of Veterinary Medicine, University of Tehran for presence of B canis. DNA from blood was extracted using DNA extraction kit and PCR test was carried
out on the samples. A (825-bp
of the gyrase) gene subunit-A was used in the PCR detection. We found no B.canis in any sample suggesting 0 prevalence in this
case.
Keywords: Brucella canis,
Brucellosis, Dog, polymerase chain reaction, electrophoresis.
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