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OJVRTM
Online Journal of Veterinary Research©
(Including
Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
29 (10): 599-605, 2025.
Differential restriction endonuclease of Brucella
abortus, suis
and melitensis
Shrikrishna I, Suryanarayana
WS, Satyanarayana Rao M, Rajasekhar
M.
Indian Veterinary
Research Institute, Izzatnagar ,Department of
Veterinary Public Health, Veterinary College, University of Agricultural Sciences,
Hebbal,
Bangalore, India
ABSTRACT
Shrikrishna I, Suryanarayana WS, Satyanarayana
Rao M, Rajasekhar M., Differential
restriction endonuclease of Brucella abortus, suis and melitensis, Onl J Vet
Res., 29 (10): 599-605, 2025. Restriction endonuclease analysis (REA) of
genomic DNA of Brucella abortus strains 99, 544 and 19, B melitensis 16 M and B melitensis Ind /JNMC/98, a biotype 1 Indian isolate and B suis 1330
was carried out. Brucella strains could not be distinguished on the basis of
REA with Eco R I, Hind III and Pvu II as these enzymes produced large number of
co-migrating fragments. Furthermore, Kpn I showed two
additional fragments of 2418 and 1860 bp in B. abortus 19 and B. suis
1330. However, digestion using Not I and Xba I
enzymes uniquely identified B. melitensis strains.
Key
words: Restriction analysis, Brucella spp.
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