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OJVRTM
Online
Journal of Veterinary Research
Volume 5:3-9, 2001. Redacted 2018.
Restriction endonuclease
analysis of selected brucella strains
Shrikrishna I,1
Suryanarayana WS,2 Satyanarayana
Rao M3, Rajasekhar M 1
1 Project Directorate on Animal Disease
Monitoring And Surveillance (PD_ADMAS), Hebbal,
Bangalore 560024, 2 National Biotechnology Centre, Indian Veterinary Research
Institute, Izzatnagar 243122, 3 Department of
Veterinary Public Health, Veterinary College, University of Agricultural
Sciences, Hebbal, Bangalore 560024, India
SUMMARY
Shrikrishna I, Suryanarayana
WS, Satyanarayana Rao M, Rajasekhar
M., Restriction endonuclease analysis of selected brucella
strains, Onl J Vet Res., 5:3-9, 2001. Restriction endonuclease analysis
(REA) of genomic DNA of Brucella abortus
strains 99, 544 and 19, B melitensis 16 M and B melitensis Ind /JNMC/98, a
biotype 1 Indian isolate and B suis 1330 was carried
out. Brucella strains could not be distinguished on the basis of REA with Eco R
I, Hind III and Pvu II
as these enzymes produced large number of co-migrating fragments. Furthermore, Kpn I showed two additional fragments of 2418 and 1860 bp in B. abortus 19 and B. suis 1330. However, digestion
using Not I and Xba I enzymes uniquely identified B.melitensis strains.
Key-words: Brucella strains, Restriction endonuclease.