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OJVRTM
Online Journal of Veterinary Research©
Volume 1:53-56, 2002. Extensively redacted 2018.
A serologic investigation of Brucella melitensis of farm animals and dogs in Southern Greece
by indirect and competitive ELISA.
Burriel AR1, Bisias
G2, Butsini S3, Kontos V4
1Laboratory of Microbiology and Parasitology, Faculty of Veterinary
Medicine, University of Thessaly, Karditsa 43100,
Greece, 2Veterinary Investigation Centre, Ministry of Agriculture, Palini, Greece, 3Institute of Infectious and Parasitic
Diseases, Laboratory of Parasitology, Athens, Greece, 4School of
Public Health, Leoforos Alexandras,
Athens , Greece kanenas@rediff.com
ABSTRACT
Burriel AR, Bisias G, Butsini S, Kontos V, Serologic study of Brucella melitensis
in farm animals and dogs in Greece by indirect and competitive ELISA.
Onl J Vet Res., 1:53-56, 2002. Cattle (277),
516 pigs, 264 sheep, 292 goats and 287 dogs serum
samples from animals with or without reproductive problems were tested for Brucella
melitensis by competitive (cELISA)
and indirect (iELISA) tests. Using both tests, bovine
samples were negative, dogs were negative to cELISA
but 2 and 7 suspects were found by rapid agglutination test to B canis and B melitensis, respectively. Fourteen pig samples
showed low association and 1 positive using cELISA. B
melitensis antibody was found in 2.3 and 9.4% of
normal sheep but 39.4 and 26% in abortive sheep using the cELISA
and iELISA, respectively. In goats, 11.3 and 19.5%
tested positive. cELISA and iELISA showed 85% and 59% agreement in positive tests from
sheep and goats, respectively. Of 779 serum samples tested with both ELISA
methods, 95 were positive with iELISA and 60 with cELISA but only 57.9% of the 95 samples were positive with
both methods. The results show that B. melitensis
in small ruminants is not yet an epidemiological threat to other farm animals
or dogs in Greece
KEY-WORDS: Serology, Brucella melitensis, ELISA.
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