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OJVRTM
Online Journal of Veterinary Research©
Volume 10 (2) : 116 -120, 2006. Redacted 2018.
Sequence characterization and phylogenetic analysis of
glucose-6-phosphate dehydrogenase gene in Bubalus bubalis
Kumar Ra,
Kaura Ra, Kaura Sa, Sethi Pb, Srikala Ra.
aDepartment of Animal Breeding and Genetics, College of Veterinary Medicine,
GADVASU, PAU Campus, Ludhiana,
India. bDepartment of Poultry Science, University of Georgia, 30602, Athens, USA
ABSTRACT
Kumar R, Kaura R, Kaura
S, Sethi P, Srikala R.,
Sequence characterization and phylogenetic analysis of glucose-6-phosphate
dehydrogenase gene in Bubalus bubalis,
Onl J Vet Res., 10
(2) : 116 -120, 2006. Glucose-6-phosphate dehydrogenase (G6PD has a crucial role in
fatty acid biosynthesis and detoxification of organic peroxides. It also
indirectly governs the de novo synthesis
of nucleotides by influencing the production of ribose-5-phosphate through the
hexose monophosphate pathway. In this study, a 683 bp
fragment of buffalo G6PD gene was amplified, sequenced and characterized. The
deduced amino acid sequence clustered the Bubalus bubalis G6PD sequence with the other
mammalian G6PD proteins into a monophyletic group. The multiple sequence
alignment of Bubalus bubalis G6PD
with the mammalian species clearly revealed conservation of the substrate and
dimer binding sites with the solved X-ray crystallographic model structure of Homo sapiens.
Keywords - Glucose-6-phosphate-dehydrogenase, Buffalo, G6PD, Bubalus bubalis).
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