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OJVRTM

Online Journal of Veterinary Research©

Volume 20(6):415-421, 2016.

 Genetic diversity of mitochondrial D-loop nucleotide sequences in Khoozestan river buffaloes

 

Hamid Reza Seyedabadi1*, Cyrus Eydivandi2, Babak Gharib2.

 

1 Department of Animal Biotechnology, Animal Science Research Institute of IRAN (ASRI), Agricultural Research Education and Extension Organization (AREEO),Karaj 2Department of animal science, College of Agriculture, Behbahan Branch, Islamic Azad University, Khoozestan, Iran. *Corresponding Author: Hamid Reza Seyedabadi

 

ABSTRACT

 

Seyedabadi HR, Eydivandi C, Gharib B., Genetic diversity of mitochondrial D-loop nucleotide sequences in Khoozestan river buffaloes, Onl J Vet Res., 20(6):415-421, 2016.  Genetic diversity and a phylogenetic tree of Khoozestan river buffaloes (40) was determined by mitochondrial DNA sequencing. Phylogenetic analysis was carried out using hyper variable region 1 (519 bp) D-loop sequences with 18 different haplotypes from 21 polymorphic sites. The 18 haplotypes are rich in A/T content as in other mammals. A phylogenetic tree was constructed from the Khoozestan buffalo sequences and representatives of published haplotypes of river and swamp buffaloes and clustering suggested common origin. Khoozestan buffalo are similar to domesticated river buffalo from other world areas.

 

Keywords: Khoozestan buffalo, mitochondrial D-loop sequence, haplotypes, phylogenetic tree.

 

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