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OJVRTM
Online Journal of Veterinary Research©
Volume
23 (8):752-762, 2019.
Effect of
ferrous ascorbate on in vitro capacitation of crossbred bull spermatozoa.
Amrit K Bansal° and Gurmail
S Bilaspuri *
Department of Animal
Breeding & Genetics, Guru Angad Dev Veterinary
& Animal Sciences University, Ludhiana-141004,
Punjab, India.
ABSTRACT
Bansal AK, Bilaspuri GS., Effect of ferrous
ascorbate on in vitro capacitation of crossbred bull spermatozoa, Onl J Vet Res., 23 (8):752-762, 2019. We
induced mild lipid peroxidation (LPO) in crossbred cattle bull spermatozoa with
ferrous ascorbate (FeAA – 150 µM FeSO4
: 750 µM ascorbic acid) to evaluate its effect on sperm capacitation and
acrosome reaction. Fresh semen samples of crossbred cattle bulls (HHS,
Holstein-Friesian x Sahiwal : FC, Friesian Crosses :
1F and 4F, 1st and 4th generation interbreed) were suspended
in TALP medium (pH 7.4). The suspension was divided into controls of TALP +
spermatozoa) and test ferrous ascorbate (FeAA -150 µM
FeSO4 : 750 µM ascorbic acid) +
spermatozoa] incubated 6hrs at 37°C and assessed for morphology, %
hyperactivity, % acrosome reaction and change in LPO level during capacitation.
Morphology of spermatozoa was not affected by FeAA with
normal capacitation and acrosome reaction. However, FeAA
reduced sperm movement and increased LPO. Results suggested that ascorbate increased
reactive oxygen species (ROS)/LPO/oxidative stress without affecting other
sperm functions.
Keywords: FeAA,
sperm capacitation, acrosome reaction, LPO, % hyperactivity.