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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 27 (9): 539-545, 2023.
Effect of Coxiella burnetii on egg embryo development and pathology.
Norina L2, Noordin MM1, Sabri MY1,
Goh MY1, Zamri-Saad M1,
Sarenasulastri AB2, Azizul
MO2, Norsyahril A2, Krishnan M2,
Salmeah AR2, Cheah
CP2.
1Faculty of Veterinary Medicine, Univerity Putra Malaysia 43400 Serdang,
Selangor, 2Malaysian Animal Health Laboratory (MALH), Bukit Tengah
14000 Bukit Mertajam, Penang.
ABSTRACT
Norina L, Noordin MM, Sabri MY, Goh MY, Zamri-Saad
M, Sarenasulastri AB, Azizul
MO, Norsyahril A, Krishnan M, Salmeah
AR, Cheah CP., Effect of Coxiella
burnetii on egg embryo development and pathology, Onl J Vet Res., 27 (9): 539-545, 2023. Tissue samples were taken from goats with history of abortion tested
for Coxiella burnetti
by stain smear confirmed by PCR. Six
samples of 15 embryonated chicken eggs each, were inoculated with 10-4, 10-5,
10-6, 10-7, 10-8 and 10-9 Coxiella burnetti. Control
eggs were inoculated PBS. Embryos were harvested by 10 days for lesion microscopy,
Gimenez stain coccobacilli confirmed by
immunochemistry. Embryos in eggs inoculated 10-4 Coxiella
burneti started to die by day 2 and by day 4 with
10-5 bacteria. There was no mortality observed from day 4 to 10. We
found no chorion-allantoic membrane lesions by day 2
to 5. Controls had clear chorion with intact normal blood vessels. In contrast
by day 5, those inoculated 10-4 Coxiella
presented cloudy membranes and albumin with white spotted foci attached to the
chorionic membrane and collapsed blood vessels. Our results were confirmed by immunohistochemistry
being very sensitive to antigen. We find that inoculation of embryonated SPF egg with Coxiella
burnetti could avoid use of
tissue or animals if carried out in Biohazard safety cabinet Class II (Esco) safety protocol.
Key
words:
Coxiella burnetii,
IHC, SPF embryonated egg, goat