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OJVRTM
Online Journal of Veterinary Research©
Volume 9(1):6-11, 2005, Redacted 2018.
Zahoor MA, Hussain I, Mahmood MS.
Molecular
Virology Laboratory, Department of Microbiology, University of
Agriculture, Faisalabad, 38040, Pakistan, Phone: +92-41-9201093, Fax:
+92-41-9200183,
zahooratif@hotmail.com
ABSTRACT
Zahoor MA,
Hussain I, Mahmood MS, One-Step RT-PCR for detection of infectious bursal
disease, Onl J Vet Res., 9(1):6-11, 2005. A simple method for the rapid
detection of infectious bursal disease virus (IBDV) was developed employing a
single-tube reverse transcription–polymerase chain reaction (RT-PCR). The
method utilized a single buffer system for both RT and PCR and was performed
without interruption as a single thermal cycling program. A set of primers was
used to amplify 743bp fragment of the VP2 gene from the nucleotides 701 to
1444. The method was applied to detect IBDV in 26 bursa samples. Twenty one
samples were found to contain the IBDV genome. The results of the present study
indicated that the single-tube RT-PCR may be a useful method for detecting IBDV
in bursa tissue samples from suspected cases in epidemiological investigations.
Key Words: IBDV;
Rapid Detection; One-Step RT-PCR; Clinical Samples