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OJVRTM
Online Journal of
Veterinary Research ©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 28 (1): 37-42,
2024.
Inhibition
of infectious bursal disease virus by Withania somnifera
Manali Pant, Umapathi V, Tanuj
Ambwani, Rashmi Singh, Anil Kumar.
Department Of Biotechnology, Graphic Era University,Dehradun, IVRI, Palampur,
Veterinary Biochemistry, ,G.B.P.U.A&T,Pantnagar, Microbiology, Mathura University, Department Of Molecular Biology and Genetic
Engineering G.B.P.U.A&T,Pantnagar, India.
ABSTRACT
Pant M, Umapathi
V, Ambwani T, Singh R, Kumar A., Inhibition of
infectious bursal disease virus by Withania somnifera, Onl J Vet Res., 28 (1): 37-42, 2024. Infectious bursal disease is a
notifiable, highly contagious viral disease of chickens mostly 3-6 weeks of
age. Effect of Withania somnifera
(WS) on replication of infectious bursal disease (IDB) virus is described. Different passages of UABz-2 strain of IBD
virus were cultured through chicken embryo fibroblast. Primer set (23 mer)
was used for amplification of 248 bp fragment of
hypervariable region of VP2 gene of IBDV6. For IBDV growth and
inhibition by WS, virus titration was performed. Antiviral activity of extract
was determined by treating virus infected CEF cells with WS and cytopathicity observed at 24 hrs
interval for three days. Reading CPE was recorded and titer calculated. Hydro
alcoholic extract of Withania somnifera inhibited
IBD virus 99.9 % at a concentration of 25µg/ml in cytopathic inhibition assays.
Findings were supported by RT-PCR.
Keywords: Infectious
Bursal Disease Virus, Ashwagandha, Hydroalcoholic extract, RT-PCR.
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