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OJVRTM
Online
Journal of Veterinary Research©
Volume 20(4):237-242, 2016.
Polymorphisms in Calpastatine
gene of Lori fat tail sheep.
Nematollah Asadi1,2,
Saber Khederzadeh3, Shahram
Nanekarani4, Narges abdali1 and
Mohammad Hussein BanabazI5
1Razi Herbal Medicines
Research Center , Lorestan University of
Medical sciences, khoramabad 2Dep.of
Biotechnology Animal Science Research Institute (ASRI) 3Natural History Museum and
Genetic Resources, Department of Environment, Pardisan Eco-Park, Tehran, 4Islamic Azad University Boroujerd Branch, Brojerd. 5Department of Animal Science, Faculty of Agricultural Science and
Engineering, College of Agriculture & Natural Resources, University of
Tehran, Karaj, Iran.
ABSTRACT
Asadi N, Khederzadeh S, Nanekarani S, Abdali N, Banabazi MH.,
polymorphisms in Calpastatine gene in Lori fat tail
sheep., Onl J Vet Res., 20(4):237-242, 2016. Calpastatin coded by a candidate gene on ovine
chromosome 5 has a regulatory role in muscle development and meat tenderness at
slaughter. Genomic
DNA was extracted from 100 whole blood samples from Lori fat tailed sheep in Lorestan, Iran. A
polymorphism within exon 1 of Calpastatin gene was
revealed by PCR-RFLP with MspI restriction enzyme. PCR amplified a 622bp
fragment consisting of exon and intron I located in L domain of calpastatin and digested as 2 fragments of 336 and 286bp.
Genotypes AA, AB and BB had frequencies of 32.2, 63.2 and 4.6, respectively. In
addition, this locus was not at Hardy–Weinberg equilibrium for Lori sheep
population.
Key words: Calpastatin Gene, Polymorphism, RFLP, Lori Sheep.
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