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OJVRTM
Online Journal of Veterinary
Research©
Volume 21(8):497-504, 2017.
Effect of cannabinoid CB-1 receptor on expression of
hypothalamic neuropeptide Y mRNA gene in male Wistar
rats
Mohammad Reza Khansari1, Negar Panahi1*,
Saeed Hosseinzadeh2, Morteza Zendehdel3,
1Department(s) of Veterinary Basic Sciences, Science and Research
Branch, Islamic Azad University, Tehran, 2Food Hygiene and Public
Health, School of Veterinary Medicine, Shiraz University, Shiraz, 3Basic
Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
ABSTRACT
Khansari MR, Panahi
N, Hosseinzadeh S, Zendehdel
M., Effect of cannabinoid CB-1 receptor on expression of hypothalamic
neuropeptide Y mRNA gene in male Wistar rats, Onl J Vet Res., 21(8):497-504, 2017. It is unclear whether cannaboids are orexigenic
or anorexigenic. Groups of 6 male Wistar
rats each were injected 2-Arachidonyl glycerol (2-AG) as CB1 receptor agonist
and rimonabant hydrochloride (SR) as receptor
antagonist. Controls were fed ad libitum.
Other groups were starved 12h or
injected 0.5, 1 and 2µg/rat
SR or 2-AG intra cerebro-ventricularly (ICV).
Neuropeptide (NPY) mRNA expression was determined through quantitative
real time PCR assay. Results show that ICV injection of 2-AG and food restriction,
induced over expression of hypothalamic NPY gene compared with controls. Expression of hypothalamic NPY gene declined after injection
with SR compared with controls. Results suggest that CB1 agonist receptor may
induce hypothalamic NPY gene
expression, whereas antagonist receptors, reduce it.
Key words: endocannabinoids, NPY mRNA expression, hypothalamus,
CB1 receptor agonist and antagonist, hypoglycemia.