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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

26 (9): 725-732, 2022.

Pathology and RT PCR of highly virulent canary pox virus

 

 Monire Khordadmehr2, Vahid Reza Ranjbar1 Azizollah Khodakaram Tafti2,3

 

1Department of Avian Medicine, 2Pathology, School of Veterinary Medicine, Shiraz University, 3Razi Vaccine and Serum Research Institute, Shiraz, Iran.

 

ABSTRACT

 

Khordadmehr M, Ranjbar VR, Tafti AK., Pathology and RT PCR of highly virulent canary pox virus, Onl J Vet Res., (9): 725-732, 2022.  We describe outbreak of poxvirus of 200 yellow breed canaries with mortality >99%. Clinical signs before death included severe conjunctivitis, dyspnea, anorexia, weakness, rapid weight loss, puffy appearance and cutaneous proliferative lesions around eyes and beaks for 5-7 days. Cutaneous lesions around eyes and beak were characterized by epidermal hypertrophy and hyperplasia, degeneration of stratified squamous epithelium with typical intracytoplasmic Bollinger bodies. In lungs we found edema, hyperemia and hemorrhages with fibrinous pneumonitis. Tissue samples of eyelids, heart, liver, skin and lungs with or without histopathological lesions were examined by PCR showing virus DNA in eyelids (12/12), skin (12/12) and lungs (10/12) by 578 base pair (bp). However, we found no virus in liver or heart.

 

Keywords: Canary, pox virus, lesions, Bollinger bodies, PCR.

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