Histopathological studies on canine mammary tumors with special reference to p53 gene mutations

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OJVRTM

Online Journal of Veterinary Research©

Volume 18(7): 528-542, 2014. Redacted 2017.

Histopathological studies on canine mammary tumors with special reference to p53 gene mutations

Hemanth I, Kumar R, Varshney KC*, Nair MG, ThanislassJ¹, SivakumarM², Ramesh KumarB³

Dept(s) of Veterinary Pathology, ¹Biochemistry, ²Anatomy & Histology ³Surgery & Radiology Rajiv Gandhi Institute of Veterinary Education and Research, Puducherry, Pondicherry, India. *Corresponding Author:

ABSTRACT

Hemanth I, Kumar R, Varshney KC, Nair MG, Thanislass J, Sivakumar M, Ramesh Kumar B., Histopathological studies on canine mammary tumors with special reference to p53 gene mutations. Onl J Vet Res., 18(7): 528-542, 2014. Canine mammary neoplasms are the most common neoplastic condition accounting for ~52% of all tumors in bitches. One-third to a half are malignant. Because tumor suppressor genes are involved in the origin and progression of human and canine neoplasms, mutational status can be used for prognosis. Histopathological grading, histochemical characteristics and incidence of p53 gene mutations from 10 canine mammary tumors is reported. Two tumors were benign and 8 malignant. Histological grading of the carcinomas showed 12.50% well differentiated grade I, 50% moderately differentiated grade II and 37.25% grade III (poorly differentiated), neoplasm PCR-SSCP analysis of DNA isolated from 10 samples for exons 5-8 of p53 gene revealed 7 mutations in 3 samples of which 3 ductal carcinomas had mutations in 4 codons 147, 148, 149 and 150 and two each had mis-sense and silent types within exon 5. Tubulo-papillary adenocarcinomas showed mutations in 2 codons 126 and 158, one each of mis-sense and silent types within exon 5. Benign mixed mammary tumor revealed single silent mutation involving the codon 188 of exon 6. Presence of p53 gene mutations in canine mammary tumor samples in our study correlated with histological aggressiveness (higher histological grades) of respective neoplasms.

Key words: Canine mammary tumors – histopathology – mutations - p53 tumor suppressor gene – Polymerase chain reaction – Single strand

conformation polymorphism.

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