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Online Journal of Veterinary Research©
Volume 16 (4):172-181, 2012. Extensively redacted 2017.

Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA and agglutination tests.

M. Khordadmehr¹, S. M. H. Hosseini²^٭, E. Mohsenifar³, M. M. Namavari², S. Khordadmehr⁴

1Departments of Pathology, 3Clinical Sciences, School of Veterinary Medicine, Shiraz University, 2. Razi Vaccine and Serum Research Institute, Shiraz, and 4. School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran,

ABSTRACT

Khordadmehr M, Hosseini SMH. Mohsenifar E, Namavari MM, Khordadmehr S., Seroprevalence of Neospora caninum in farm and household dogs determined by ELISAand agglutination test, Online J Vet Res., 16 (4):172-181, 2012. Neospora caninum is an important cause of abortion in dairy cattle worldwide. The dog is the definitive host for N. caninum which also infects dairy cattle. We report seroprevalence of Neospora caninum infection in dogs in Fars, Iran. Serum samples (180) were collected from dogs of different sex, age, and breed and subjected to a modified agglutination (MAT) and standardized dot-ELISA tests. Fifty nine samples (54.62%) were positive for N. caninum antibody by dot-ELISA and 49 samples (44.44%) were positive by MAT in titers of 1:100 and 1:80, respectively. There were no significant differences in serological values between different groups of age, sex, household or farm dogs. There was 79.8% agreement between dot-ELISA and MAT tests.

Keywords: Neospora caninum . Dot- ELISA. Modified agglutination test

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