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OJVRTM
Online Journal of Veterinary Research©
Volume 16 (2):72-80, 2012

In vitro cultivation of Besnoitia caprae

 

Namavari M1,*, Namazi F2, Oryan A2, Mansourian M1, Rahimian A1, Tahamtan Y1

 

 

1DVM, PhD. Razi Vaccine and Serum Research Institute, Shiraz, Iran, 2DVM, PhD. Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

 

ABSTRACT

 

Namavari M, Namazi F, Oryan A, Mansourian M, Rahimian A, Tahamtan Y., In vitro cultivation of Besnoitia caprae, Online J Vet Res, 16 (2):72-80, 2012. Different cell lines were inoculated with tachyzoites of Besnoitia caprae (BC-Pars isolate) obtained from mice including Vero, MDCK and BHK21. The best growth of tachyzoites was observed in BHK21. No replication of parasites was seen on MDCK and Vero cell. Mice and chicken embryonated eggs were inoculated with tachyzoites obtained from BHK21 cell culture. Skin samples from the mice and chicken infected with tachyzoites of BC-Pars were PCR positive. Histopathological sections of their skin revealed presence of necrosis and mononuclear cells infiltration. To authors knowledge, this is the first report of successful in vitro B. caprae cell culture. The impact of B. caprae developing in cell culture is very significant and will facilitate many aspects of Besnoitia research.

 

Key words: Besnoitia caprae; Cell culture; BALB/c mice; Chicken emryonated eggs.

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