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OJVRTM
Online Journal of
Veterinary Research©
Volume 13 (1):41-52, 2009. Editors redaction 2017.
Characterization of caprine (Capra hircus) SLC11A1 gene: innate resistance to paratuberculosis
Liandris E1, Gazouli M2,
Ikonomopoulos J1
1Agricultural
University of Athens, Faculty of Animal Science, Laboratory of Anatomy-
Physiology and 2University of Athens, School of
Medicine, Laboratory of Biology, Athens, Greece
ABSTRACT
Liandris E, Gazouli M, Ikonomopoulos J, Characterization of the caprine (Capra hircus) SLC11A1 gene:innate
resistance to paratuberculosis,
Onl J Vet Res, 13 (1):41-52, 2009. Control
of paratuberculosis by current means is considered a
demanding task. Recent advances in immunology have permitted the identification
of genes, amongst which SLC11A1 (solute carrier
family 11 member A1), is associated with
resistance or sensitivity to specific diseases. The aim of this study was to
determine the sequence and structure of caprine (Capra hircus)
SLC11A1 gene and the
association of its polymorphisms with innate resistance to Mycobacterium avium subsp. paratuberculosis (MAP). One sample of blood, serum and
faeces was collected from each of 50 goats over the age of 2, from a herd of
400 adult animals with a long record of paratuberculosis.
This material was used for the sequence and structure analysis of the SLC11A1
gene and the detection of MAP DNA and antibody using Real Time PCR and ELISA.
The goat SLC11A1 gene coding sequence consisted of 2004 bases and presented a
1648-bases Open Reading Frame encoding a 548 residue protein. The 3'
untranslated region of the targeted gene revealed 2 microsatellites consisting
of a variable number of guanine-thymine repeats, named regions A and B.
Statistical analysis of the results indicated a significant level of
association between the B8 allele of the polymorphic region B and
the presence of MAP DNA in the
faeces. The results presented here suggest association of specific
polymorphisms of the SLC11A1 gene with innate resistance/sensitivity of goats to paratuberculosis is possible.
Key words: slc11a1 and paratuberculosis in goats.
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