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OJVRTM
Online
Journal of Veterinary Research©
(Including Medical and
Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (5): 300-305, 2025.
Electrometric cholinesterase
method for plasma, brain and liver dichlorvos and carbaryl.
Fouad KM, Banan Al-Baggou
,College of Veterinary Medicine, University of Mosul, Mosul, Iraq
Abstract
Fouad KM , Al-Baggou B., Electrometric cholinesterase method for plasma, brain and liver dichlorvos
and carbaryl., Onl J Vet
Res., 29 (5):300-305, 2025. We describe an
electrometric method for cholinesterase (ChE) in plasma,
brain and liver of 45 days old chickens given oral organophosphate insecticides.
ChE assay contained 0.2ml plasma and tissue
homogenate, 3ml water, 3ml phosphate-barbital buffer pH 8.1 and 0.12ml 7.5%
acetyl-thiocholine iodide substrate for 30min incubation
at 37 ˚C. ChE in the plasma was 0.47, brain 0.25
and liver 0.21 ΔpH/30min. Oral administration of 7mg/kg dichlorvos, and 100mg/kg carbamate induced cholinergic
overstimulation, associated with significant (P < 0.01) decreases of 29-62%
in plasma ChE and 28 and 65% in brain tissue.
KEY-WORDS: Cholinesterase, Chicken, Dichlorvos, Carbaryl,
Organophosphate, Carbamate.
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