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    Online Journal of Veterinary Research © 

  15 (1): 31-37, 2011.  Redacted 2017.

Genotyping Alpha S1 Casein (Exon 10 and 11) Gene by PCR-SSCP in Sahiwal Cattle

 

Lydia Dhanammal1  PhD., Ishwar Dayal Gupta2 PhD., Sathyanarayana Rachagani2 PhD., and Archana Verma2 PhD.,

                                                                        

1Rajiv Gandhi College of Veterinary and Animal Sciences, Pondicherry-605009, 2Animal Genetics and Breeding, Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, Haryana-132001, India

 

 ABSTRACT

 

Dhanammal L, Gupta Id, Rachagani S, Verma A., Genotyping Alpha S1 Casein (Exon 10 and 11) Gene by PCR-SSCP in Sahiwal Cattle. Onl J Vet Res, 15 (1): 31-37, 2011. Casein genetic polymorphisms are important due to their effects on quantitative traits and technological properties of milk. In this study, alpha S1 casein (CSN1S1) gene (Exon 10 and 11) were typed by polymerase chain reaction single strand conformation polymorphism (PCR-SSCP) in 168 Sahiwal cattle. Five SSCP gel patterns were observed for which the PCR products were sequenced. Based on quality screening the 314 bp fragment size decreased to 201 bp fragment size containing three nucleotide variants at 98.21 %, 1.19 % and 0.06 % of the population. A non-synonymous SNP was identified at exon 11 (A→T) at position +11462 of CSN1S1 resulting in a deduced amino acid exchange Glu(A)→Asp(T), and found in 1.19 % of the population. The second SNP was identified at intron 11 (G→A). Sahiwal is an indigenous cattle breed and the presence of a non-synonymous SNP is indicative of its unique genomic architecture.

 

KEYWORDS: Casein genes, PCR-SSCP, Sahiwal cattle, SNP’s, genetic polymorphism, gel electrophoresis

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