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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (10): 606-611, 2025.
Caprine chromosomal DNA of Corynebacterium
pseudotuberculosis,
Mohan P, Jayaprakasan V, Punnoose KT, Mini A.
Project Directorate on Animal Disease
Monitoring And Surveillance, Hebbal,
Bangalore – and Department of Microbiology,
College of Veterinary and Animal Sciences, Kerala Agricultural University, India
SUMMARY
Mohan P, Jayaprakasan
V, Punnoose KT, Mini A..
Chromosomal DNA of goat Corynebacterium
pseudotuberculosis, Onl J Vet Res., 29 (10): 606-611,
2025. Corynebacterium pseudotuberculosis from goat caseous lymphadenitis samples
were evaluated against standard reference strains by biochemical and
restriction endonuclease analysis (REA) of chromosomal DNA. The
enzymes Eco R1, Bgl II, Pst1 and Bam H1 were used to
characterize the organisms. Both bacteria were similar except for
one isolate which fermented lactose. REA of both organisms yielded different
restriction patterns digested with Pst
I but not with Eco RI, Bam HI or Bgl II. Both strains
were nitrate negative. A 3.2-Kb fragment was absent in 4 enzymes whereas 6.6-Kb
and 3.6-Kb DNA fragments were found in both strains. The results suggest a
high degree of homogeneity between field isolates and reference strains of C.
pseudotuberculosis (goat).
Key words: Corynebacterium pseudotuberculosis, lymphadenitis - caprine -
restriction endonuclease analysis.
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