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Online Journal of Veterinary Research©
Volume 5:14-18, 2001. Redacted 2018.
Biochemical and restriction enzyme analysis of
Corynebacterium pseudotuberculosis (goat).
Mohan P1*,
Jayaprakasan V2, Punnoose
KT 2, Mini A2.
1 Research Associate, Project Directorate on Animal
Disease Monitoring And Surveillance (PD_ADMAS), IAH & VB Campus, Hebbal, Bangalore – 560
024, INDIA. 2Department of Microbiology, College of
Veterinary and Animal Sciences, Kerala Agricultural University, Mannuthy, Thrissur, Kerala, INDIA. *Corresponding
author
SUMMARY
Mohan P, Jayaprakasan V Punnoose
KT, Mini A., Biochemical and restriction enzyme analysis of
Corynebacterium pseudotuberculosis (goat), Onl J Vet
Res., 5:14-18, 2001. Corynebacterium
pseudotuberculosis from goat caseous
lymphadenitis samples were evaluated against standard reference strains by
biochemical and restriction endonuclease analysis (REA) of chromosomal
DNA. The enzymes Eco R1, Bgl II, Pst1 and
Bam H1 were used to characterize the organisms. Both bacteria were similar except for one isolate which
fermented lactose. REA of both organisms yielded different restriction patterns
digested with Pst I but not
with Eco RI, Bam HI or Bgl II. Both strains were
nitrate negative. A 3.2-Kb fragment was absent in 4 enzymes whereas 6.6-Kb and
3.6-Kb DNA fragments were found in both strains. The results suggest a high degree of homogeneity between field isolates
and reference strains of C. pseudotuberculosis (goat).
Key words: Corynebacterium pseudotuberculosis,
lymphadenitis - caprine - restriction endonuclease analysis.