1994-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store
these pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR publications. This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors.
OJVRTM
Online Journal of Veterinary
Research©
Volume 22 (7):619-628, 2018.
Comparative pathological and molecular study of cholera induced by
ingestion of human feces in rats, mice and rabbits.
Muna Sachit
Hashim1, Suad Abdul Kareem Mohammed2,
Ali Jasim Jafer3,
Afaf Abdulrahman Yousif4,
Thikra Abdulla Mahmood5.
1,2,4College of Veterinary
Medicine, University of Baghdad; 3College of Veterinary Medicine, Karbala
University; 5Dep. of Community Medicine, College of Medicine, Kufa University, Iraq. Sachitmuna@yahoo.com.;Afaf_a.rahman@yahoo.com.
Hashim MS, Mahmood TA, Kareem Mohammed
SA, Abdurrahman A., Comparative
pathological and molecular study of cholera induced by ingestion of human feces
in rats, mice and rabbits, Onl J Vet Res., 22 (7):619-628, 2018. Ingestion of Vibrio cholera causes acute diarrhea responsible for ~100,000’s deaths per year
worldwide and extensively studied in human patients. However, there is limited
data on effect of oral infection with human material with vibrio cholera on pathology and immunology and/or DNA damage in
reproductive organs in laboratory animals. We infected orally groups of 30
rats, mice and rabbits each with 0.01mg\10gm BW feces with certified Vibrio cholerae from hospitalized patients suffering from cholera, daily for 21 days.
Controls (n=30) were given distilled water. As animals died, liver, kidney,
lung, brain, intestine and associated lymph nodes, testes and uterus were
excised. Twenty percent of infected animals survived and these were also
sacrificed for same tissue samples. Histo-immuno-tests showed
necrotic foci with hemorrhagic enteritis; liver necrosis; kidney abscesses and
brain degeneration with increased expression of CD4 and CD8 markers. Molecular
analysis using FISH technique in testes and uterus tissue sections showed DNA
damage as mutations in gene TK (11qE2)/XY.
Key words: Cholera; Molecular-Pathogenesis; Rats; Mice;
Rabbits.
FULL-TEXT
(SUBSCRIBE OR PURCHASE TITLE $25USD)