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OJVRTM

 Online Journal of Veterinary Research©

 

Volume 18(6): 489-494, 2014. Redacted 2017.


Characterization and sequencing of MT ND5 gene in native Khorasan poultry

 

Ayeh Sadat Sadr, Mohammad Reza Nassiri*

 

Department of Animal Science, International Branch of Ferdowsi University of Mashhad, Iran

 

ABSTRACT

 

Sadr AS, Nassiri MR., Characterization and sequencing of MT ND5 gene in native poultry, Onl J Vet Res., 18(6): 489-494, 2014. Mutations in mitochondria (mtDNA) can affect the incidence and severity of disease and abnormalities in vertebrates.  Characterization and sequencing of the ND5 gene in Khorasan native poultry is reported. After extraction, two DNA fragments were amplified by a specific primer, cloned to vector pTZ57R/T and transformed to an E. coli - DH5α strain. Recombinant plasmids were extracted from positive colonies and sequenced. Results showed maximum similarity with Gallus Gallus mitochondrial genome and a protein BLAST query showed that the sequence had a similarity of 93% in 95% coverage to NADH dehydrogenase subunit 5. The protein BLAST query suggested the conserved domain as NADH5 C and Oxidored q1, in a family which is part of a complex I. This catalyzes the transfer of two electrons from NADH to ubiquinone in a reaction associated with proton translocation across the membrane. The findings may be useful in identifying phenotypes in poultry.

 

Key words: Cloning, Sequencing, MT-ND5 gene, Khorasan native poultry.


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