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OJVRTM
Online Journal of Veterinary Research©
Volume 23 (11):1104-1118,
2019.
Effect of chronic high or low dose verapamil
with or without colchicine on serum
and tissue P-glycoprotein and brain histology in Albino
mice.
Tayseer Shaker Mahmood, Zainab Ismail Ibrahim, Duraid
Abdul-Hadi Abass, Zainab
Jamal Mohammad Jawad.
Department(s) of Physiology,
Biochemistry & Pharmacology and Pathology, College of Veterinary Medicine,
University of Baghdad, Iraq.
ABSTRACT
Mahmood
TS, Ibrahim ZI, Abdul-Hadi Abass
D, Mohammad Jawad ZJ., Effect of chronic high or low dose verapamil with or
without colchicine on serum and tissue P-glycoprotein and brain histology in
albino mice, Onl J Vet Res., 23 (11):1104-1118, 2019. Groups
of 7 albino mice each were gavaged 50 and 70 µg/kg
colchicine with or without
1.5 and 3mg/kg verapamil daily for 90 days. Controls (n=7) were gavaged distilled water. At day 90, heart blood was taken
for serum P-glycoprotein determined by ELISA and brain tissue excised for
microscopy and P-glycoprotein. Tissue and serum P-glycoprotein decreased 8-24%
and 16-28% respectively, with or without verapamil. However, high doses of
colchicine with verapamil had no effect on serum P-glycoprotein. High dose
colchicine induced mild congestion of blood vessels in meninges and arachinoid plexus, chromatolysis
of purkinje cells with mild cytotoxic edema and
proliferation, aggregation of oligodendrocytes and mild degeneration of
neurons. Low dose verapamil produced lesions similar to those of colchicine but
high doses induced severe chromatolysis of purkinje cells. At lower doses, the combined drugs produced
hypertrophy and dilation of vessels with congestion, proliferation and
aggregation of astrocytes and oligodendrocytes. At higher doses combined drugs micrographs
revealed severe chromatolysis, astrocytosis,
degeneration, necrosis and de-myelination.
Keywords:
Histopathology of brain tissue, colchicine, ELISA test, P-glycoprotein level. Raw
data provided.