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OJVRTM

Online Journal of Veterinary Research©

Volume 20(8):557-562, 2016

Effect of concentration, volume and temperature of serum complement of common carp (Cyprinus carpio),

 on unsensitized rabbit red blood cell hemolysis.

 

Azadeh Yektaseresht DVM, PHD1*, Amin Gholamhosseini DVM, PHD2, Ali Janparvar1, Reza Salighehzadeh DVM, PHD2

 

*1Department of Pathobiology, 2Aquatic diseases,  School of Veterinary Medicine, Shiraz University, Shiraz; Iran.

 

Yektaseresht A, Gholamhosseini A,  Janparvar A,  Salighehzadeh R., Effect of concentration, volume and temperature of serum complement of common carp (Cyprinus carpio), on unsensitized rabbit red blood cell hemolysis. Onl J Vet Res., 20(8):557-562, 2016. Red blood cell (RBC) hemolysis assay for humans has recently been adapted to assess innate immune activity in fish. The alternative pathway activity of complement system can be measured by serum hemolytic activity activated in foreign red blood cells. Effect of concentration, volume and temperature of serum complement of common carp (Cyprinus carpio), on unsensitized rabbit red blood cell hemolysis is described. Serum  concentrations of 12.5% v/v produced 3.9±0.05mm, 25%, 4.1±0.115mm, 50%, 4.9±0.05 mm and 100%, 6.05±0.05mm hemolysis. 10μL volume of serum resulted in 4.15±0.12mm, 20ul, 5.9±0.1mm and 30μL 6.2±0.23mm hemolysis. Incubation of sera at 4°C produced 6±0.08mm,  25°C, 6.25±0.19mm but at 37°C 5.9±0.05mm hemolysis.

 

Key words: Common carp, serum complement , Hemolytic assay.

 

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