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OJVR
Online Journal of Veterinary Research©
Volume 14 (2):121-142, 2010. Redacted extensively 2017.
Autologous
osteoblast and cortico-cancellous bone scaffold for
repair of compact bone critical sized
defect
in normal and osteoporotic rabbit models.
Kamran
Kaveh DVM, Rashid Ibrahim DVM PhD, Tengku Azmi Ibrahim DVM PhD,
Mohd Zuki Abu Bakar
DVM PhD.
Faculty of Veterinary Medicine,
University of Putra Malaysia 43400 UPM, Serdang,
Selangor, Malaysia.
ABSTRACT
Kamran Kaveh K, Ibrahim R,
Ibrahim TA, Abu Bakar MZ, Autologous osteoblast and cortico-cancellous
bone scaffold for repair of compact bone critical sized defect in normal and
osteoporotic rabbit models Onl J Vet Res, 14 (2):121-142,
2010. We describe bone scaffold of autologous osteoblasts
with cortico-cancellous bone for repairing critical
sized defects of compact bone in normal and osteoporotic white New Zealand
rabbit models. One group of 6 control rabbits were
implanted with the cortico-cancellous bone scaffold
only. Bone marrow-derived mesenchymal stem cell from 6 rabbits were cultured,
differentiated into osteoblasts and seeded into the scaffold of the cortico-cancellous bone scaffold. This scaffold was then
implanted in 6 normal and 6 osteoporotic rabbits. Radiographs were taken for 8
weeks to evaluate osteogenicity. Rabbits were
euthanized at week 8 and implants harvested for gross, histological and
scanning electron microscope observations. In those implanted scaffold alone, creeping bone substitution at
margins and osteogenesis at the centre of the defect
with osteo-conduction and induction. In rabbits with
osteoporosis, new bone penetrated through very thin trabeculae throughout the
entire defect. However, new bone formation pattern appeared osteoporotic. Results suggest that the cortico-cancellous bone
scaffold with autogenous marrow-derived osteoblasts formed bone more
efficiently than scaffold alone. New bone regeneration and complete bone
healing in normal and pathologic bone occurred only at 8 weeks suggesting the
scaffold may be useful for bone repair.
Keywords: Bone tissue engineering, corticocancellous
bone scaffold, critical sized defect, osteoblasts, osteoporosis, rabbit.
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