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Online Journal of Veterinary Research ©

Volume 14 (2): 253-259, 2010.

Putative Oligosacharyl Transferase from Trypanosoma brucei rhodesiense is conserved in Family Trypanosomatidae

Waren N. Baticados^1*(DVM, PhD), Noboru Inoue²(DVM, PhD), Chihiro Sugimoto³(DVM, PhD), Hideyuki Nagasawa²(DVM, PhD) and Abigail M. Baticados¹ (DVM)

¹Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines 4031., ²National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 8555, Japan and ³Research Center for Zoonosis Control, Hokkaido University N18-W9, Kita-ku, Sapporo, Hokkaido 060-0818, Japan

ABSTRACT

Baticados WN, Inoue N, Sugimoto C, Nagasawa H, Baticados AM., Putative Oligosacharyl Transferase from Trypanosoma brucei rhodesiense is conserved in Family Trypanosomatidae, Online J Vet Res., 14 (2): 253-259, 2010. Current reports on the putative oligosaccharyl transferase (OST) from Trypanosoma brucei rhodesiense indicated that it is evolutionarily conserved within family trypanosomatidae. The study therefore endeavored to test the assertion by attempting to molecularly clone the putative gene in other trypanosome species. An amplicon size of ~ 2000 bp were amplified in Trypanosoma congolense and Trypanosoma evansi using primers for cloning Trypanosoma brucei rhodesiense putative OST. The PCR bands were akin to reported amplicon size of Trypanosoma brucei rhodesiense putative OST. The data provides evidence that the putative OST gene reported in Trypanosoma brucei rhodesiense was truly evolutionarily conserved in family Trypanosomatidae. The study additionally indicated that this gene is not only conserved between species of the same genus but also between subgenera of genus Trypanosoma.

Key words: N-glycosylation, PCR, Protozoa, Trypanosomatidae, Trypanosomes

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